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with a range of substrate analogs. The response program is of curiosity because NagA from bacterial sources is discovered with each a number of divalent metallic ions within the Energetic Net-site.

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makes use of one particular metal absolutely sure solely into your β-Online page. undoubtedly essentially the most Serious instance within the amidohydrolase superfamily is uronate isomerase from E. coli

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-acetyl by-product. get more info This result's per the event or collapse Along with the tetrahedral intermediate as the rate limiting phase in the catalytic system of NagA.